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Competing Lipid-Protein and Protein-Protein Interactions Determine Clustering and Gating Patterns in KcsA. Molina, M.L., Giudici, A.M., Poveda, J.A., Fernández-Ballester, G., Montoya, E., Renart, M.L., Fernández, A.M., Encinar, J.A., Riquelme, G., Morales, A., & González-Ros, J.M. 2015. J Biol Chem. 290(42): 25745-25755.


  1. Doyle, D. A., Morais, C. J., Pfuetzner, R. A., Kuo, A., Gulbis, J. M., Cohen, S. L., Chait, B. T., and MacKinnon, R. (1998) The structure of the potassium channel: molecular basis of K+ conduction and selectivity. Science 280, 69-77.
  2. Zhou, Y., Morais-Cabral, J. H., Kaufman, A., and MacKinnon, R. (2001) Chemistry of ion coordination and hydration revealed by a K+ channel-Fab complex at 2.0 A resolution. Nature 414, 43-48.
  3. Uysal, S., Vasquez, V., Tereshko, V., Esaki, K., Fellouse, F. A., Sidhu, S. S., Koide, S., Perozo, E., and Kossiakoff, A. (2009) Crystal structure of full-length KcsA in its closed conformation. Proc. Natl. Acad. Sci. U. S. A 106, 6644-6649.
  4. Schrempf, H., Schmidt, O., Kummerlen, R., Hinnah, S., Muller, D., Betzler, M., Steinkamp, T., and Wagner, R. (1995) A prokaryotic potassium ion channel with two predicted transmembrane segments from Streptomyces lividans. EMBO J. 14, 5170-5178.
  5. Meuser, D., Splitt, H., Wagner, R., and Schrempf, H. (1999) Exploring the open pore of the potassium channel from Streptomyces lividans. FEBS Lett. 462, 447-452.
  6. Splitt, H., Meuser, D., Borovok, I., Betzler, M., and Schrempf, H. (2000) Pore mutations affecting tetrameric assembly and functioning of the potassium channel KcsA from Streptomyces lividans. FEBS Lett. 472, 83-87.
  7. Heginbotham, L., Kolmakova-Partensky, L., and Miller, C. (1998) Functional reconstitution of a prokaryotic K+ channel. J. Gen. Physiol 111, 741-749.
  8. LeMasurier, M., Heginbotham, L., and Miller, C. (2001) KcsA: it's a potassium channel. J. Gen. Physiol 118, 303-314.
  9. Chakrapani, S., Cordero-Morales, J. F., and Perozo, E. (2007) A quantitative description of KcsA gating II: single-channel currents. J. Gen. Physiol 130, 479-496.
  10. Zakharian, E. and Reusch, R. N. (2004) Streptomyces lividans potassium channel KcsA is regulated by the potassium electrochemical gradient. Biochem. Biophys. Res. Commun. 316, 429-436.
  11. Zakharian, E. and Reusch, R. N. (2004) Functional evidence for a supramolecular structure for the Streptomyces lividans potassium channel KcsA. Biochem. Biophys. Res. Commun. 322, 1059-1065.
  12. Molina, M. L., Barrera, F. N., Fernandez, A. M., Poveda, J. A., Renart, M. L., Encinar, J. A., Riquelme, G., and Gonzalez-Ros, J. M. (2006) Clustering and coupled gating modulate the activity in KcsA, a potassium channel model. J. Biol. Chem. 281, 18837-18848.
  13. Hegermann, J., Overbeck, J., and Schrempf, H. (2006) In vivo monitoring of the potassium channel KcsA in Streptomyces lividans hyphae using immuno-electron microscopy and energy-filtering transmission electron microscopy. Microbiology 152, 2831-2841.
  14. Valiyaveetil, F. I., Zhou, Y., and MacKinnon, R. (2002) Lipids in the structure, folding, and function of the KcsA K+ channel. Biochemistry 41, 10771-10777.
  15. Demmers, J. A., van Dalen, A., de Kruijff B., Heck, A. J., and Killian, J. A. (2003) Interaction of the K+ channel KcsA with membrane phospholipids as studied by ESI mass spectrometry. FEBS Lett. 541, 28-32.
  16. Deol, S. S., Domene, C., Bond, P. J., and Sansom, M. S. (2006) Anionic phospholipid interactions with the potassium channel KcsA: simulation studies. Biophys. J. 90, 822-830.
  17. Lee, A. G. (2004) How lipids affect the activities of integral membrane proteins. Biochim. Biophys. Acta 1666, 62-87.
  18. Cheng, M. H., Xu, Y., and Tang, P. (2009) Anionic lipid and cholesterol interactions with alpha4beta2 nAChR: insights from MD simulations. J. Phys. Chem. B 113, 6964-6970.
  19. Fersht, A. R. (1999) Structure and mechanism in protein science. A guide to enzyme catalysis and protein folding, 3rd Ed., New York.
  20. Marius, P., Alvis, S. J., East, J. M., and Lee, A. G. (2005) The interfacial lipid binding site on the potassium channel KcsA is specific for anionic phospholipids. Biophys. J. 89, 4081-4089.
  21. Triano, I., Barrera, F. N., Renart, M. L., Molina, M. L., Fernandez-Ballester, G., Poveda, J. A., Fernandez, A. M., Encinar, J. A., Ferrer-Montiel, A. V., Otzen, D., and Gonzalez-Ros, J. M. (2010) Occupancy of nonannular lipid binding sites on KcsA greatly increases the stability of the tetrameric protein. Biochemistry 49, 5397-5404.
  22. Barrera, F. N., Renart, M. L., Poveda, J. A., de, K. B., Killian, J. A., and Gonzalez-Ros, J. M. (2008) Protein self-assembly and lipid binding in the folding of the potassium channel KcsA. Biochemistry 47, 2123-2133.
  23. Marius, P., de Planque, M. R., and Williamson, P. T. (2012) Probing the interaction of lipids with the non-annular binding sites of the potassium channel KcsA by magic-angle spinning NMR. Biochim. Biophys. Acta 1818, 90-96.
  24. van Dalen, A., Hegger, S., Killian, J. A., and de Kruijff, B. (2002) Influence of lipids on membrane assembly and stability of the potassium channel KcsA. FEBS Lett. 525, 33-38.
  25. Molina, M. L., Encinar, J. A., Barrera, F. N., Fernandez-Ballester, G., Riquelme, G., and Gonzalez-Ros, J. M. (2004) Influence of C-terminal protein domains and protein-lipid interactions on tetramerization and stability of the potassium channel KcsA. Biochemistry 43, 14924-14931.
  26. Giudici, A. M., Molina, M. L., Ayala, J. L., Montoya, E., Renart, M. L., Fernandez, A. M., Encinar, J. A., Ferrer-Montiel, A. V., Poveda, J. A., and Gonzalez-Ros, J. M. (2013) Detergent-labile, supramolecular assemblies of KcsA: relative abundance and interactions involved. Biochim. Biophys. Acta 1828, 193-200.
  27. Schagger, H. and von Jagow, G. (1991) Blue native electrophoresis for isolation of membrane protein complexes in enzymatically active form. Anal. Biochem. 199, 223-231.
  28. Schagger, H., Cramer, W. A., and von Jagow, G. (1994) Analysis of molecular masses and oligomeric states of protein complexes by blue native electrophoresis and isolation of membrane protein complexes by two-dimensional native electrophoresis. Anal. Biochem. 217, 220-230.
  29. Neuhoff V, Stamm R, and Eibl H (1985) Clear background and highly sensitive protein staining with Coomassie Blue dyes in polyacrylamide gels: a systematic analysis. Electrophoresis 6, 427-448.
  30. Riquelme, G., Lopez, E., Garcia-Segura, L. M., Ferragut, J. A., and Gonzalez-Ros, J. M. (1990) Giant liposomes: a model system in which to obtain patch-clamp recordings of ionic channels. Biochemistry 29, 11215-11222.
  31. Hamill, O. P., Huguenard, J. R., and Prince, D. A. (1991) Patch-clamp studies of voltage-gated currents in identified neurons of the rat cerebral cortex. Cereb. Cortex 1, 48-61.
  32. Chiantia, S., Kahya, N., and Schwille, P. (2005) Dehydration damage of domain-exhibiting supported bilayers: an AFM study on the protective effects of disaccharides and other stabilizing substances. Langmuir 21, 6317-6323.
  33. Jiang, Y., Lee, A., Chen, J., Cadene, M., Chait, B. T., and MacKinnon, R. (2002) Crystal structure and mechanism of a calcium-gated potassium channel. Nature 417, 515-522.
  34. Thompson, J. D., Higgins, D. G., and Gibson, T. J. (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22, 4673-4680.
  35. Schwede, T., Kopp, J., Guex, N., and Peitsch, M. C. (2003) SWISS-MODEL: An automated protein homology-modeling server. Nucleic Acids Res. 31, 3381-3385.
  36. Guex, N. and Peitsch, M. C. (1997) SWISS-MODEL and the Swiss-PdbViewer: an environment for comparative protein modeling. Electrophoresis 18, 2714-2723.
  37. Laskowski, R. A., Rullmannn, J. A., MacArthur, M. W., Kaptein, R., and Thornton, J. M. (1996) AQUA and PROCHECK-NMR: programs for checking the quality of protein structures solved by NMR. J. Biomol. NMR 8, 477-486.
  38. Molina, M. L., Barrera, F. N., Encinar, J. A., Renart, M. L., Fernandez, A. M., Poveda, J. A., Santoro, J., Bruix, M., Gavilanes, F., Fernandez-Ballester, G., Neira, J. L., and Gonzalez-Ros, J.M. (2008) N-type inactivation of the potassium channel KcsA by the Shaker B "ball" peptide: mapping the inactivating peptide-binding epitope. J. Biol. Chem. 283, 18076-18085.
  39. Krieger, E., Koraimann, G., and Vriend, G. (2002) Increasing the precision of comparative models with YASARA NOVA - a self-parameterizing force field. Proteins 47, 393-402.
  40. Duan, Y., Wu, C., Chowdhury, S., Lee, M. C., Xiong, G., Zhang, W., Yang, R., Cieplak, P., Luo, R., Lee, T., Caldwell, J., Wang, J., and Kollman, P. (2003) A point-charge force field for molecular mechanics simulations of proteins based on condensed-phase quantum mechanical calculations. J. Comput. Chem. 24, 1999-2012.
  41. Cao, Y., Jin, X., Huang, H., Derebe, M. G., Levin, E. J., Kabaleeswaran, V., Pan, Y., Punta, M., Love, J., Weng, J., Quick, M., Ye, S., Kloss, B., Bruni, R., Martinez-Hackert, E., Hendrickson, W. A., Rost, B., Javitch, J. A., Rajashankar, K. R., Jiang, Y., and Zhou, M. (2011) Crystal structure of a potassium ion transporter, TrkH. Nature 471, 336-340.
  42. Guerois, R., Nielsen, J. E., and Serrano, L. (2002) Predicting changes in the stability of proteins and protein complexes: a study of more than 1000 mutations. J. Mol. Biol. 320, 369-387.
  43. Schymkowitz, J., Borg, J., Stricher, F., Nys, R., Rousseau, F., and Serrano, L. (2005) The FoldX web server: an online force field. Nucleic Acids Res. 33, W382-W388.
  44. Cordero-Morales, J. F., Jogini, V., Chakrapani, S., and Perozo, E. (2011) A multipoint hydrogen-bond network underlying KcsA C-type inactivation. Biophys. J. 100, 2387-2393.
  45. Weingarth, M., Prokofyev, A., van der Cruijsen, E. A., Nand, D., Bonvin, A. M., Pongs, O., and Baldus, M. (2013) Structural determinants of specific lipid binding to potassium channels. J. Am. Chem. Soc. 135, 3983-3988.
  46. Raja, M. and Vales, E. (2009) Effects of sodium chloride on membrane fusion and on the formation of aggregates of potassium channel KcsA in Escherichia coli membrane. Biophys. Chem. 142, 46-54.
  47. Seeger, H. M., Bortolotti, C. A., Alessandrini, A., and Facci, P. (2009) Phase-transition-induced protein redistribution in lipid bilayers. J. Phys. Chem. B 113, 16654-16659.
  48. Heuberger, E. H., Veenhoff, L. M., Duurkens, R. H., Friesen, R. H., and Poolman, B. (2002) Oligomeric state of membrane transport proteins analyzed with blue native electrophoresis and analytical ultracentrifugation. J. Mol. Biol. 317, 591-600.
  49. Nicke, A., Rettinger, J., Mutschler, E., and Schmalzing, G. (1999) Blue native PAGE as a useful method for the analysis of the assembly of distinct combinations of nicotinic acetylcholine receptor subunits. J. Recept. Signal. Transduct. Res. 19, 493-507.
  50. Vinothkumar, K. R., Raunser, S., Jung, H., and Kuhlbrandt, W. (2006) Oligomeric structure of the carnitine transporter CaiT from Escherichia coli. J. Biol. Chem. 281, 4795-4801.
  51. McCormick, J. I. and Johnstone, R. M. (1988) Volume enlargement and recovery of Na+-dependent amino acid transport in proteoliposomes derived from Ehrlich ascites cell membranes. J. Biol. Chem. 263, 8111-8119.
  52. Cordero-Morales, J. F., Cuello, L. G., Zhao, Y., Jogini, V., Cortes, D. M., Roux, B., and Perozo, E. (2006) Molecular determinants of gating at the potassium-channel selectivity filter. Nat. Struct. Mol. Biol. 13, 311-318.
  53. Blunck, R., Cordero-Morales, J. F., Cuello, L. G., Perozo, E., and Bezanilla, F. (2006) Detection of the opening of the bundle crossing in KcsA with fluorescence lifetime spectroscopy reveals the existence of two gates for ion conduction. J. Gen. Physiol 128, 569-581.
  54. Heginbotham, L., LeMasurier, M., Kolmakova-Partensky, L., and Miller, C. (1999) Single Streptomyces lividans K+ channels: functional asymmetries and sidedness of proton activation. J. Gen. Physiol 114, 551-560.
  55. Imai, S., Osawa, M., Mita, K., Toyonaga, S., Machiyama, A., Ueda, T., Takeuchi, K., Oiki, S., and Shimada, I. (2012) Functional equilibrium of the KcsA structure revealed by NMR. J. Biol. Chem. 287, 39634-39641.
  56. Marius, P., Zagnoni, M., Sandison, M. E., East, J. M., Morgan, H., and Lee, A. G. (2008) Binding of anionic lipids to at least three nonannular sites on the potassium channel KcsA is required for channel opening. Biophys. J. 94, 1689-1698.
  57. Iwamoto, M. and Oiki, S. (2013) Amphipathic antenna of an inward rectifier K+ channel responds to changes in the inner membrane leaflet. Proc. Natl. Acad. Sci. U. S. A 110, 749-754.
  58. Dibb, K. M., Rose, T., Makary, S. Y., Claydon, T. W., Enkvetchakul, D., Leach, R., Nichols, C. G., and Boyet, M. R. (2003) Molecular basis of ion selectivity, block, and rectification of the inward rectifier Kir3.1/Kir3.4 K+ channel. J. Biol. Chem. 278, 49537-49548.
  59. Undrovinas, A. I., Fleidervish, I. A., and Makielski, J. C. (1992) Inward sodium current at resting potentials in single cardiac myocytes induced by the ischemic metabolite lysophosphatidylcholine. Circ. Res. 71, 1231-1241.
  60. Goforth, R. L., Chi, A. K., Greathouse, D. V., Providence, L. L., Koeppe, R. E., and Andersen, O.S. (2003) Hydrophobic coupling of lipid bilayer energetics to channel function. J. Gen. Physiol. 121, 477-493.
  61. Botelho, A. V., Huber, T., Sakmar, T. P., and Brown, M. F. (2006) Curvature and hydrophobic forces drive oligomerization and modulate activity of rhodopsin in membranes. Biophys. J. 91, 4464-4477.
  62. Grage, S. L., Keleshian, A. M., Turdzeladze, T., Battle, A. R., Tay, W. C., May, R. P., Holt, S. A., Contera, S. A., Haertlein, M., Moulin, M., Pal, P., Rohde, P. R., Forsyth, V. T., Watts, A., Huang, K. C., Ulrich, A. S., and Martinac, B. (2011) Bilayer-mediated clustering and functional interaction of MscL channels. Biophys. J. 100, 1252-1260.
  63. Spira, F., Mueller, N. S., Beck, G., von Olshausen, P., Beig, J., and Wedlich-Soldner, R. (2012) Patchwork organization of the yeast plasma membrane into numerous coexisting domains. Nat. Cell Biol. 14, 640-648.
  64. Mueller, N. S., Wedlich-Soldner, R., and Spira, F. (2012) From mosaic to patchwork: matching lipids and proteins in membrane organization. Mol. Membr. Biol. 29, 186-196.